Sat Sep 13, 2014 10:58 am
It depends on how you treat/rinse/clean your yeast. I've ventured into 20+ territory. It can be done. Rinsing correctly & nutrient are key along with the standards of good cleaning & sanitation. Harvesting from a solid, controlled ferment is pretty important too.
The fail safe I used was to harvest, rinse & separate into 2 cultures. Grow them both back up & repeat. You'll have several branches going from the original strain. Some aren't going to do well & you get rid of them. I came up with a system to keep track of which branch & generation each strain was, just to keep them all straight.
If you're looking for the easy way to do it, buy a new vial every couple of gens.
Lee
"Show me on this doll where the internet hurt you."
"Every zoo is a petting zoo if you man the fuck up."
BN Army // 13th Mountain Division