Posted: Thu May 25, 2006 9:00 am
Honestly, I haven't been reading this thread, but this post caught my eye. Enzymes do not reactivate.
While this is true, Kai was worried about continued enzymatic activity while still in the temperature range that alpha/beta amylase are still active. I don't worry about it either, and thought I made myself clear on that subject. Guess I better be clearer on my intent in the future
Once they are denatured, that's it. It is exactly the same process that turns an egg from clear and runny to white and hard. Cooling down a cooked egg doesn't make it slimy again. Neither will cooling the wort reactivate enzymes. Anyway, it wouldn't really matter if they did reactivate. Once conversion is complete, it's complete; extra time or even extra enzymes won't change the wort makeup significantly.
While you are essentially correct, given your assumption that Kai has gone beyond the temperature stability of amylase, he said that he hadn't. I thoght I had told him not to worry about it as even if there were any enzymatic activity by that time that it would be trivial and therefore moot. At any rate, relax, don't worry about it and just make great beer bro!
Prost!
Michel
While this is true, Kai was worried about continued enzymatic activity while still in the temperature range that alpha/beta amylase are still active. I don't worry about it either, and thought I made myself clear on that subject. Guess I better be clearer on my intent in the future
Once they are denatured, that's it. It is exactly the same process that turns an egg from clear and runny to white and hard. Cooling down a cooked egg doesn't make it slimy again. Neither will cooling the wort reactivate enzymes. Anyway, it wouldn't really matter if they did reactivate. Once conversion is complete, it's complete; extra time or even extra enzymes won't change the wort makeup significantly.
While you are essentially correct, given your assumption that Kai has gone beyond the temperature stability of amylase, he said that he hadn't. I thoght I had told him not to worry about it as even if there were any enzymatic activity by that time that it would be trivial and therefore moot. At any rate, relax, don't worry about it and just make great beer bro!
Prost!
Michel